Composite

Part:BBa_K3002113:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


Level 1 – MHETase + cCA + HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1327
    Illegal PstI site found at 1651
    Illegal PstI site found at 1994
    Illegal PstI site found at 2804
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1327
    Illegal PstI site found at 1651
    Illegal PstI site found at 1994
    Illegal PstI site found at 2804
    Illegal NotI site found at 1662
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2572
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1327
    Illegal PstI site found at 1651
    Illegal PstI site found at 1994
    Illegal PstI site found at 2804
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1327
    Illegal PstI site found at 1651
    Illegal PstI site found at 1994
    Illegal PstI site found at 2804
    Illegal NgoMIV site found at 1260
    Illegal NgoMIV site found at 1721
    Illegal NgoMIV site found at 1751
    Illegal NgoMIV site found at 2066
    Illegal NgoMIV site found at 2084
    Illegal NgoMIV site found at 2120
    Illegal NgoMIV site found at 2763
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.



Source

This part is a combination of the parts BBa_K3002027 (PAR-promoter), BBa_K3002006 (RPL23-Terminator), BBa_K3002037 (CDS of MHETase), BBa_K3002007 (cCA), BBa_K3002017 (HA-tag), BBa_K3002302 (connector B1-B2), BBa_K3002303 (connector B2-B3), BBa_K3002304 (connector B4-B5) and BBa_K3002305 (connector B5-B6).

References